Figure 6.

Intracellular dialysis with TUDCA activates Cl^– currents. Representative whole‐cell currents recorded during intracellular dialysis with TUDCA (100 μM) by inclusion in the patch pipette (A). Currents measured at –40 mV (○), representing I_Cl– are shown. Horizontal bar above the trace indicates application of the Ca²⁺ ionophore, ionomycin (1 μM). Area between brackets is shown in expanded view (bottom). Representative traces with inclusion of EGTA (10 mM/L) in pipette (B) and in the presence of the ATP hydrolyzing enzyme apyrase (5 U/mL) in the bath solution (C). (D) Cumulative data showing the magnitude of TUDCA‐stimulated currents, reported as current density (‐pA/pF) under control conditions and with intracellular Ca²⁺ chelation (EGTA in pipette) or apyrase in the bath solution. ^**Intracellular delivery of TUDCA significantly increases currents (n = 13; P < 0.05). ^*Currents were inhibited by EGTA (n = 9, P < 0.01) or apyrase (n = 10, P < 0.01).