Figure 7.

The transmembrane protein TENM2 is a novel target gene of ZEB1. (a) Representation of ZEB1 binding to the TENM2 gene; ChIP–Seq peaks (marked in red box) and tracks of H3K27Ac ChIP–Seq, which is used as a marker of enhancer activity, based on data available in the UCSC genome browser. (b) ChIP–qPCR showing significant enrichment of the TENM2 promoter region in a ChIP experiment using the ZEB1 antibody, relative to enrichment by non‐specific IgG. (c) Measurement of relative amount of TENM2 mRNA expressed in C3 and CZ1 Hs578T cells after normalization with the HPRT1 house‐keeping mRNA. (d) Kaplan–Meier plot of DMFS of patients (n = 28) belonging to the triple‐negative breast cancer group, relative to the probability of survival. Significant difference is shown for two groups of patients classified based on high and low levels of TENM2 mRNA expression obtained from GOBO and graphed using the Kaplan–Meier plotter tool. Statistical significance p‐value <0.01; n = 3