Figure 4.

Biocatalyzed reactions with continuous substrate feeding (CSF) showing concentration of substrate (black squares) and product (red circles) as a function of time with a feed rate of (a) 10 mmol L^‐1 h^‐1 over 5 h, (b) 20 mmol L^‐1 h^‐1 over 3 h. Black dashed lines indicate the substrate feeding rate, and black dotted lines indicate the sum of substrate and product concentrations. Reactions conditions: 10% v v^‐1 methanol, [TmCHMO‐PTDH] = 4 µmol L^‐1, [NADP⁺] = 250 µmol L^‐1. Below: biphasic biocatalyzed oxidation with toluene with (c) substrate concentration and substrate conversion (red dashed line), and (d) production concentration as a function of time. Concentrations in the toluene phase are shown with empty symbols, concentrations in the water phase with black symbols and the sum of the concentrations with green triangles. Reactions conditions: [substrate] = 100 mmol L^‐1 in toluene (33% v v^‐1), [TmCHMO‐PTDH] = 4 µmol L^‐1, [NADP⁺] = 250 µmol L^‐1.