Table 1.
Primary and Secondary Antibodies
| Antibodies | Species | Type | Dilution | Source |
|---|---|---|---|---|
| Ionized calcium‐binding adapter molecule 1 (IBA‐1) | Rabbit | Monoclonal IgG | 1:300 | Wako, Japan |
| Neurofilament 200 (NF) | Rabbit | Polyclonal IgG | 1:500 | Merck Millipore, Billerica, USA |
| Glial fibrillary acidic protein (GFAP) | Mouse | Monoclonal IgG | 1:300 | Sigma, St. Louis, USA |
| Myelin basic protein (MBP) | Mouse | Monoclonal IgG | 1:500 | Merck Millipore, Billerica, USA |
| Neurotrphin 3 (NT‐3) | Rabbit | Polycolonal IgG | 1:300 | Sigma, USA |
| Cy3 conjuncted anti rabbit secondary antibody | Goat | Polyclonal IgG | 1:600 | Jackson ImmunoResearch, West Grove, USA |
| FITC conjuncted anti mouse secondary antibody | Goat | Polyclonal IgG | 1:50 | Jackson ImmunoResearch, West Grove, USA |