Figure 5.

Replication levels of RNA1 and RNA2 in insect‐derived and plant‐derived Rice stripe virus (RSV) in rice (Oryza sativa Huangjinqing). (a) The RNA levels of viral RNA1 and RNA2 relative to that of rice ubiquitin in rice leaves within 5 d after inoculation with insect‐derived or plant‐derived RSV as measured by quantitative real‐time PCR. The values represent the means ± SEs from eight replicates. Student's t‐tests were performed to evaluate the differences between two means at the same time point. **, P < 0.01. (b) Accumulations of RNA1 and RNA2 with the extended 3′‐terminal sequences (3′‐ETS) in rice leaves after inoculation with insect‐ or plant‐derived RSV as measured with touchdown reverse transcription‐polymerase chain reaction (RT‐PCR). The total amounts of viral RNA1 and RNA2 and the transcripts of rice actin were amplified as internal controls.