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. 2018 Jun 20;245(4):456–467. doi: 10.1002/path.5099

Figure 3.

PATH-5099-FIG-0003-b

Loss of α‐catenin induces anoikis resistance in mouse and human E‐cadherin‐expressing cell lines and apoptosis in E‐cadherin‐mutant mouse ILC cells. Anoikis resistance was determined in mouse (A) and human (B) breast cancer cell lines upon α‐catenin iKD by FACS analysis using binding to annexin‐V and propidium iodide. Note the reversal to anoikis sensitivity upon reconstitution of α‐catenin in the mouse Trp53Δ/Δ cell lines (+ Dox Rescue; red bars). (C–E) Loss of α‐catenin in mouse ILC cells leads to apoptosis. Western blot (C) showing the extent of α‐catenin knockdown in two independent mouse ILC cells and the induction of cleaved caspase‐3 expression (c‐Caspase 3). Akt was used as loading control. Phenotypical consequences are shown in D. Note the apoptotic cells in the Dox‐treated cells (arrow). (E) Anoikis resistance was determined in the mILC cell lines upon α‐catenin iKD. Error bars represent the standard deviation of three independent experiments. **p = 0.001.