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. 2018 Jun 1;219(3):1124–1133. doi: 10.1111/nph.15229

Figure 6.

Figure 6

Pigment identification. (a) Absorbance spectra derived from different coloured petal pieces (yellow or orange) immersed in refractive index (RI) oil with = 1.42. (b) Absorbance spectra taken from ethanol extracts from white, yellow or orange petal pieces. The three‐peaked absorbance spectra are a clear signature of carotenoid pigments. (c) Raman spectra from pigment extracts upon excitation with a 532 nm laser with c. 1 mW power. The peaks characteristic for carotenoids are marked. The shift of the Raman peak to shorter wavenumbers for the orange extract is indicative of a longer carotenoid chain.