Table 2.
Possible identities of EV components targeted by IgG antibodies following vaccination
| Accession number | Protein | Band 1 | Band 2 | Band 3 | Mw (kDa) |
|---|---|---|---|---|---|
| aTMUE_s0245000500 | VWD and vitellogenin N and DUF1943‐domain‐containing protein | 9 | 17 | 190 | |
| aTMUE_s0093001800 | Vacuolar protein sorting‐associated protein 52 | 5 | 4 | 164 | |
| aTMUE_s0070003500 | TSP‐1 domain‐containing protein | 3 | 39 | ||
| aTMUE_s0189001400 | Neurogenic locus notch protein | 3 | 53 | ||
| TMUE_s0015006300 | Peptidase M8 domain‐containing protein | 4 | 60 | ||
| TMUE_s0037004100 | Conserved hypothetical protein | 4 | 51 | ||
| aTMUE_s0022000400 | Na+ K+ ATPase alpha subunit 1 | 3 | 118 | ||
| TMUE_s0117002800 | Trypsin and CUB domain‐containing protein | 3 | 69 | ||
| TMUE_s0093001400 | Nicastrin family protein | 3 | 78 | ||
| TMUE_s0106000600 | Moesin:ezrin:radixin 1 | 3 | 64 | ||
| TMUE_s0011007600 | Anoctamin | 2 | 96 | ||
| TMUE_s0060000200 | Prominin domain‐containing protein | 2 | 75 | ||
| TMUE_s0059000500 | Neurogenic locus notch protein | 2 | 62 | ||
| TMUE_s0320000100 | Neurogenic locus notch protein 2 | 2 | 49 |
Bands corresponding to 100 (Band 1), 80 (Band 2) and 70 kDa (Band 3) were excised from the SDS‐PAGE gel shown in Figure 3D, and their protein content was analysed by mass spectrometry. The proteins identified within these bands are listed. The number of unique peptides identified for each protein is displayed (criteria set to 95% protein threshold, 50% peptide threshold).
a
Indicates proteins identified within ES depleted of EVs (as reported in14).