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Kinetics of spectral shifts in test media and buffered riboflavin solution. Formation/degradation kinetics of the cluster 1/cluster 2 region in EEM landscape plots of (a) CDPM and (b) DMEM. (c) Spectral change in Rf fluorescence properties (5 µ mol L−1 solution in isotonic PBS pH 7.1) upon light irradiation under the standardized experimental setup. Spectra were recorded at hourly intervals over a total light exposure time of 0–8 h.
