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. 2018 Jun 1;93(8):2141–2151. doi: 10.1002/jctb.5643

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© 2018 The Authors. Journal of Chemical Technology & Biotechnology published by JohnWiley & Sons Ltd on behalf of Society of Chemical Industry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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JCTB-5643-FIG-0007-b — Analysis of IgG formation in CHO shake flask cultures in light‐irradiated CDPM. (a) Intracellular IgG was detected via fluorescence‐immunostaining in fixed and permeized cells with subsequent flow cytometric analysis (mean cell‐associated fluorescence intensity; closed bars), extracellular IgG was quantified by HPLC (open bars). (b) Proportional composition of the CHO cell population at different light exposure times. For each time point, cells were stratified according to the intracellular IgG level (high/medium‐high/medium/medium‐low/low intracellular IgG staining).