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. 2018 Jun 1;93(8):2141–2151. doi: 10.1002/jctb.5643

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© 2018 The Authors. Journal of Chemical Technology & Biotechnology published by JohnWiley & Sons Ltd on behalf of Society of Chemical Industry.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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JCTB-5643-FIG-0008-c — Fluorescence micrograph of intracellular IgG localization in CHO cells. Cells from shake flask cultivation (0 h LET, end of exponential phase) were fixed, permeized, and stained with the FITC‐labeled anti‐IgG antibody (FITC‐anti‐IgG). Alexa594‐conjugated wheat germ agglutinin (aWGA) was used as a Golgi marker, and nuclei were labeled with a DNA‐specific stain (DAPI). Note the high degree of co‐localization between IgG and Golgi compartments (arrows), which is clearly visible in the morphological overlay with differential interference contrast (Overlay + DIC).