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. 2018 Apr 6;48(7):1120–1136. doi: 10.1002/eji.201847483

Figure 5.

Figure 5

ZIKV NS5 blocks type I IFN signaling. (A) Stable HEK293‐ISRE‐reporter cells were transfected with ZIKV plasmids as indicated and, 24 h later, were treated or were not with 20U/ml IFN‐A/D. Luciferase activity in cell lysates was determined 24 h after transfection and was set to 1 in untreated cells that received empty vector. (B,C) The experiment shown in (A) was repeated using plasmid combinations as indicated on the left. (D,E) HEK293T cells were transiently transfected with the indicated ZIKV plasmids, a plasmid encoding F‐Luc under ISRE control and a plasmid expressing Renilla luciferase (R‐Luc) from the TK promoter. Cells were treated with IFN‐A/D after 24 h as indicated. F‐Luc and R‐Luc activities in cell lysates were determined after an additional 24 h and the F‐Luc / R‐Luc ratio was set to 1 in untreated cells that received empty vector. Panels (A), (D), (E) are representative of three independent experiments (average and SD (n = 3)). Panels (B) and (C) show pooled data from three experiments (average and SEM (n = 9)). (A), (D), (E): unpaired, two‐tailed t‐test as indicated. (B), (C): unpaired, two‐tailed t‐test vs empty vector. (* p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001).