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. Author manuscript; available in PMC: 2018 Jul 23.
Published in final edited form as: JAMA Neurol. 2013 Sep 1;70(9):1140–1149. doi: 10.1001/jamaneurol.2013.3499

Table 2. Autoantibody Characteristics of Patients With SPS, Epilepsy, and T1DM.

Patient No.   Diagnosis GAD Autoantibody Titer, U/mL   IHC GAD Autoantibody KD, nmol WB rhGAD65, IOD % IgG Binding, Scorea
EGFP-Expressing Neurons GABA-Expressing Neurons EGFP/GABA-Negative Neurons
  1 C-SPS 700 000 Positive 0.13 95.3 3 4 1
  2 C-SPS 180 000 Positive 0.54 ND 2 4 0
  3 C-SPS   75 000 Positive 0.13 ND 1 3 0
  4 C-SPS   50 000 Positive 0.33 80.2 2 2 0
  5 C-SPS   34 000 Positive 0.43 56.7 1 0 0
  6 C-SPS   21 324 Positive 0.13 80.5 2 2 0
  7 C-SPS   12 500 Positive 0.28 51.6 2 1 0
  8 C-SPS      7500 Positive 0.33 38.1 ND ND ND
  9 C-SPS      4800 Positive 0.78 40.4 ND ND ND
10 C-SPS      3600 Negative 0.33 13.6 0 0 0
11 C-SPS        889 Negative 0.06 3.8 0 0 0
12 C-SPS        836 Negative 0.14 ND 0 0 0
13 SLS      5682 Positive 0.17 59.6 2 2 0
14 SLS        676 Negative 0.14 13.4 ND ND ND
15 SLS        405 Negative 0.65 19.2 1 2 1
16 SLS          <1 Negative ND 0 0 0
17 SLS          <1 Negative ND 0 1 0
18 J-SPS       4200 Negative 0.28 26.8 0 0 0
19 J-SPS            1 Negative ND 0 0 0
20 J-SPS            4 Negative ND 0 0 0
21 A-SPS   80 000 Positive 0.74 88.4 ND ND ND
22 A-SPS   17 000 Positive 0.17 14.2 ND ND ND
23 A-SPS          <1 Negative ND 0 0 0
24 A-SPS          <1 Negative ND 0 0 0
25 PERM       5451 Positive 0.17 ND 0 1 0
30 LADA        285 Negative 0.68 10.3 ND ND ND
31 T1DM          72 Negative 0.34 7.3 ND ND ND
32 T1DM          72 Negative 0.13 ND ND ND ND
33 T1DM          60 Negative 0.31 5.9 ND ND ND
34 T1DM          12 Negative 0.15 6.8 ND ND ND

Abbreviations: EGFP, enhanced green fluorescent protein; GABA, γ-aminobutyric acid; GAD, glutamic acid decarboxylase; IHC, immunohisto-chemistry; IOD, integrated optical densities of immunoreactive bands on Western blotting of recombinant human GAD65 (rhGAD65) expressed as a percentage of the IOD of GAD monoclonal antibody; KD, antibody dissociation constant; ND, not determined; WB, Western blot. Other abbreviations: See Table 1.

a

Serum antibodies binding to surface determinants of unpermeabilized primary neurons in culture were scored as 0 (none), 1 (1%-20% of total GABAergic neurons), 2 (21%-40%), 3 (41%-60%), or 4 (>60%).