Table 1. Primer sequences.

Gene of interest	Sequence	Amplicon size (bp)	Accession #	Annealing temperature (˚C)
MC2R	F: 5′-GAGAACCTGTTGGTGGTGGT-3′	105	EU119870.1	60
	R: 5′-GAGGGAGGAGATGGTGTTGA-3′
StAR	F: 5′-TGGGGAAGGTGTTTAAGCTG-3′	101	AB047032.2	60
	R: 5′-AGGGTTCCAGTCTCCCATCT-3′
P450scc	F: 5′-GCTTCATCCAGTTGCAGTCA-3′	140	S57305.1	60
	R: 5′-CAGGTCTGGGGAACACATC-3′
EF1α	F: 5′-CATTGACAAGAGAACCATTGA-3′	95	AF498320.1	56
	R: 5′-CCTTCAGCTTGTCCAGCAC-3′
18sRNA	F: 5′-TGCGGCTTAATTTGACTCAACA-3′	117	AF243428.2	55
	R: 5′-CAACTAAGAACGGCCATGCA-3′

Forward (F) and reverse (R) sequences, amplicon size, accession number and annealing temperature for the primers used in real-time quantitative PCR.