Table 3. Liver metabolic capacity in rainbow.
| Treatment | Post-stress enzyme activity (μmol min-1 mg-1 protein) |
Time effect (p<0.05) | ||||
|---|---|---|---|---|---|---|
| Pre-stress | 1 h | 4 h | 24 h | |||
| LDH | Control | 143.38 ± 2.57 | 145.05 ± 3.11 | 149.78 ± 5.20 | 135.68 ± 7.33 | 24 h < 4 h |
| TFM | 143.53 ± 4.16 | 143.52 ± 4.60 | 144.85 ± 3.31 | 133.43 ± 2.35 | 24 h < 4 h | |
| HK | Control | 3.55 ± 0.10 | 3.66 ± 0.12 | 3.75 ± 0.12 | 3.39 ± 0.12 | |
| TFM | 3.84 ± 0.18 | 3.88 ± 0.21 | 3.69 ± 0.13 | 3.43 ± 0.10 | ||
| GK | Control | 3.29 ± 0.14 | 3.51 ± 1.17 | 3.94 ± 0.20 | 3.48 ± 0.13 | |
| TFM | 3.55 ± 0.19 | 3.76 ± 0.21 | 3.65 ± 0.16 | 3.39 ± 0.07 | ||
| PK | Control | 13.38 ± 1.32 | 13.48 ± 0.86 | 12.93 ± 1.09 | 12.95 ± 1.02 | |
| TFM | 13.27 ± 1.12 | 13.99 ± 1.08 | 13.00 ± 1.19 | 13.65 ± 1.13 | ||
| PEPCK | Control | 1.26 ± 0.05 | 1.35 ± 0.04 | 1.34 ± 0.04 | 1.30 ± 0.04 | |
| TFM | 1.32 ± 0.06 | 1.37 ± 0.04 | 1.37 ± 0.05 | 1.33 ± 0.03 | ||
| AlaAT | Control | 19.32 ± 1.85 | 19.56 ± 1.62 | 17.34 ± 1.12 | 20.19 ± 1.88 | |
| TFM | 20.38 ± 1.27 | 21.80 ± 0.79 | 18.89 ± 1.49 | 19.51 ± 0.88 | ||
| AspAT | Control | 16.06 ± 1.09 | 15.06 ± 0.87 | 14.11 ± 1.10 | 13.45 ± 0.88 | 24 h < 0 h |
| TFM | 16.26 ± 1.47 | 16.00 ± 1.18 | 14.91 ± 1.06 | 12.89 ±0.65 | 24 h < 0 h | |
Enzyme activities were measured in trout pre- (0 h) and post-exposure (1,4, 24 h) to an acute handling stressor, after recovery in clean water from either control (no TFM) or TFM treatment (nominal concentration of 7.6 mg L-1). Data presented as mean ± S.E.M. (n = 11–13; p<0.05; nested-model ANOVA with the Bonferroni correction). Lactate dehydrogenase (LDH), hexokinase (HK), glucokinase (GK), pyruvate kinase (PK), phosphoenylpyruvate carboxykinase (PEPCK), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT). N = 11–13 individual fish per measurement.