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. 2018 Aug;24(8):1428–1434. doi: 10.3201/eid2408.171454

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Molecular identification of Diphyllobothrium nihonkaiense species by restriction fragment length polymorphism analysis of PCR-amplified cox1 gene fragments, Japan, 2012–2016. Number above each lane indicates the number of proglottids in the sample. A) Digestion of cox1 gene fragments (249 bp, arrow) with AgeI. The leftmost lane is a mock digested sample. D. nihonkaiense cox1 gene did not get cut by the AgeI enzyme. B) Digestion of cox1 gene fragments with BspHI. The 2 arrows indicate the DNA fragments (164 bp and 85 bp) resulting from the digestion. M, marker.