Table. Diagnostic tests for Brucella canis in dogs*.
| Test type | Antigen detected or target DNA | Sensitivity, % | Specificity, % | Reference |
|---|---|---|---|---|
| Serologic | ||||
| Rapid slide agglutination | Cell wall | 50–75 | 83.34–99.7 | (19) |
| 2-mercaptoethanol rapid slide agglutination | Cell wall | 31.76–70 | 100 | (19) |
| Agar-gel immunodiffusion, cell wall antigen | LPS, outer membrane protein | 27.98–52.94 | 100 | (19) |
| ELISA | LPS or CPAg | 88–97 | 94.3–96.7 | (20) |
| Immunochromatographic |
R-LPS with outer membrane proteins |
89.58 |
100 |
(21,22) |
| Other | ||||
| PCR (ITS66 and ITS279) | 16S-23S rRNA gene | 100 | 86.45–100 | (23) |
| PCR (JPF/JPR) | Outer membrane protein 2 | 16.67 (whole blood); 92.31 (vaginal swab sample) | 100 (whole blood); 51.92 (vaginal swab sample) | (24) |
*CPAg, cytoplasmic protein antigen; JPF, forward primer; JPR, reverse primer; LPS, lipopolysaccharide; R-LPS, rough LPS.