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Expression of the MPT-64 recombinant protein. Distinct band around 24 kDa was observed in Lane 5, after 3 h induction with IPTG 1 mM. Lane 1: Before induction with IPTG (T0), Lane 2: After 1 h induction with IPTG (T1), Lane 3: Supernatant, after 3 h induction with IPTG, Lane 4: After 2 h induction with IPTG (T2), Lane 5: After 3 h induction with IPTG (T3), and Lane 6: Protein ladder (Fermentas, #SM0661
