Table 2.
Sequences of PCR primers
| Target gene | Primer name | Sequences (5´→3´) | GenBank accession No. |
|---|---|---|---|
| β-actin | Forward primer | GACCCTGAAGTACCCCATTGAG | Z70044 |
| Reverse primer | TTGTAGAATGTGTGGTGCCAGAT | ||
| NKp30 | Forward primer | ACTGCTGCCTTCTTGCCGTGTT | ENSCAFG00000000524 |
| Reverse primer | ACTCCAGCATCACAGTCTTGGATGT | ||
| NKp44 | Forward primer | ATCGAGTGGCAGGGCAGACA | ENSF00000008847 |
| Reverse primer | TTCCTCCTTCAGACCAATCATGGT | ||
| NKG2D | Forward primer | ACGAAGGCAAAAGAGAAAGCC | XM_849013 |
| Reverse primer | TGATGATTATGGCACCGCAT | ||
| 2B4 | Forward primer | TGGTAACGTGAGCTATGCTTGGTA | ENSF00000008741 |
| Reverse primer | ACGATCACCAGAAAGTACACGAAGT | ||
| CD16 | Forward primer | ACATTCCAGCAGCAACAAGTGA | XM_536141 |
| Reverse primer | AGCAAAATACAGCCCAGTGTCCA | ||
| CD56 | Forward primer | TTGTCCCCAGCCAAGGAGAAAT | NM_001010950 |
| Reverse primer | TAGATGGTGAGCGTGGAGGAAGA | ||
| CD160 | Forward primer | CATCCACATCCACAGCTCAATG | XM_540276 |
| Reverse primer | CGAAGACCTGTCCTTGCACAAA | ||
| Ly49 | Forward primer | TTTCCAGTGCCAAGACATCTCA | AY191818 |
| Reverse primer | CCCAGGTGATGGTTTTCTTGAA | ||
| CD94 | Forward primer | ATTCAGCCAACATTGTCACCAG | DQ228356 |
| Reverse primer | AGTAGCAGTTGCATTGGTGCC | ||
| Granzyme B | Forward primer | GCAGGAACATTCTCAACCCCA | ENSCAFG00000025287 |
| Reverse primer | TTCCAGAGACAGCCTTTTGCC | ||
| Perforin | Forward primer | GCCAACCCTTCATCAGAATTCA | ENSF00000003552 |
| Reverse primer | CACCAATTATCTCGGAAAAGCG |