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. 2018 Jul 19;11:4177–4187. doi: 10.2147/OTT.S172713

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© 2018 Ge et al. This work is published and licensed by Dove Medical Press Limited

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The expression of DcR3 in gastric cancer cell lines and generation of DcR3 knockdown cell lines.

Notes: (A) The expression of DcR3 in different GC cells was measured by enzyme-linked immunosorbent assay and quantified by RT-PCR. (B) Images captured by fluorescence microscope showing the fluorescent signal of the enhanced green fluorescent protein in stably transfected cells. (C and D) HGC27 and MKN28 cells of all groups were harvested for DcR3 measurement by Western blotting and RT-PCR. Expression of DcR3 was decreased significantly in LV-SiDcR3 group compared with control group and LV-NC group (P<0.05). ***P<0.001.

Abbreviations: DcR3, decoy receptor 3; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RT-PCR, reverse transcriptase polymerase chain reaction; LV-NC, lentiviral negative control.