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. 2018 Jul 19;11:4177–4187. doi: 10.2147/OTT.S172713

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© 2018 Ge et al. This work is published and licensed by Dove Medical Press Limited

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Cell mobility was inhibited after transfection with DcR3 siRNA in HGC27 and MKN28 cell lines.

Notes: (A) Representative set of images showing the migration of HGC27 cells toward scratch wound. Graph presenting the normalized scratch width relative to control group at 0 and 24 hours after the scratch, the pairwise comparisons between LV-SiDcR3 and control and LV-NC groups were statistically significant (P<0.05). (B) Cell invasion was determined using Transwell assay. Cell invasion was significantly inhibited after transfection with siRNA in HGC27 and MKN28 cells. (C) The wound healing assay showed cell migration was inhibited after transfection with siRNA in MKN28 cells. (D) Cell migration was determined using Transwell assay. Cell migration was significantly inhibited after transfection with siRNA in HGC27 and MKN28 cells. (E and F) DcR3 overexpression in HGC27 and MKN28 cells caused morphological alteration resembling EMT, while knockdown of DcR3 attenuated the morphological alteration. ***P<0.001.

Abbreviation: DcR3, decoy receptor 3; LV-NC, lentiviral negative control.