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. Author manuscript; available in PMC: 2018 Jul 23.
Published in final edited form as: Nat Chem Biol. 2017 Jul 24;13(9):982–993. doi: 10.1038/nchembio.2436

Figure 6. Identification of compounds with dual targets.

Figure 6

(a) Compounds predicted to target multiple distinct bioprocesses. Nodes indicate a predicted gene target located within a biological process-enriched network cluster defined in the global genetic interaction profile similarity network. Edges represent compounds predicted to target two distinct biological processes. NPD5925 was predicted to target the distinct processes of DNA catabolic process and fungal-type cell wall biogenesis (yellow edge). NP214 was predicted to target DNA replication and cellular proton transport (white node, yellow edge). (b) Measurement of cell leakage (adenylate kinase assay) from cells treated with DMSO, hydroxyurea, echinocandin B, and NPD5925 (n=3, mean ± S.E.). (c) Images of a cell stained with NPD5925 (fluorescent), DAPI, and the merged fluorescent signal. (d) Cell cycle analysis of cells following treatment with α-factor, DMSO, hydroxyurea (HU), MMS, and. NPD5925.