Table 2.

List of primer sequences for quantitative real-time PCR.

Gene symbol	AC/No	Primer sequences	Product length (bp)	Annealing Temp. (°C)
Efna5	NM_207654.2	F: 5′-TAGTTACAGGTTTGTGGGTTCG-3′ R: 5′- GGTGTTTCATCATTGGTTAGAG-3′	230	61
Epha5	NM_007937.3	F: 5′- GCTGGTTCCCATTGGGAAAT-3′ R: 5′- CACATTGGTGACTGGAGAAGGA-3′	95	57.1
Epha3	NM_010140.3	F: 5′-TCACACTGCGGGACTGTAAC-3′ R: 5′-TTCAGAATGCGATCCCCGAG-3′	187	58.7
Epha8	NM_007939.2	F: 5′- CTCAAACCAGGCACTCGCTA-3′ R: 5′- CGATGGTCCGAGTGTCGTAG-3′	127	60.5
Ephb2	NM_001290753.2	F: 5′-ACCTCAGTTCGCCTCTGTGAA-3′ R: 5′- GGCTCACCTGGTGCATGAT-3′	77	59
Pcna	NM_011045.2	F: 5′- GTGGATAAAGAAGAGGAGGCG-3′ R: 5′-TGTAGGAGACAGTGGAGTGGC-3′	111	60.5
Bax	NM_007527	F: 5′- GCCTCCTCTCCTACTTCGG-3′ R: 5′-AAAAATGCCTTTCCCCTTC-3′	187	58.5
Bcl2	NM_009741	F: 5′-TCTCTCGTCGCTACCGTCG-3′ R: 5′- CCCAGTTCACCCCATCCCT-3′	123	58
Caspase8	NM_009812.2	F: 5′-ACTCGGCCACAGGTTACGG-3′ R: 5′-TGTGTGGGATGTAGTCCAAGC-3′	137	61.6
Gdf9	NM_008110	F: 5′- CGGCTCCATCGCTTACAAA-3′ R: 5′- CTTCCCCCGCTCACACAGT-3′	187	55.1
Bmp15	NM_009757	F: 5′- GAAAATGGTGAGGCTGGTAA-3′ R: 5′- GATGAAGTTGATGGCGGTAA-3′	152	57.7
β-actin	NM_ 007393.5	F: 5′- CACGATGGAGGGGCCGGACTCATC-3′ R:5′-TAAAGACCTCTATGCCAACACAGT-3′	241	59