Figure 4. Frequency of hemifusion (measured as DiD fluorescence dequenching) as a function of number of bound Alexa-fluor-555/3-110-22 molecules.

Histogram shows the number of particles with a particular number of bound fluorescent inhibitor molecules, in bins of five. The number of particles in a bin that proceeded to hemifusion in the time of the experiment is shown as the height of the purple bar; the number of non-fusing particles is the total height of the bar (blue).