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. 2018 Jul 9;7:e32744. doi: 10.7554/eLife.32744

Figure 2. Measurement of Rad52-Rad51 interaction in cells that express various Rad52-FLAG mutants.

Figure 2.

(A) Rad51 immunoprecipitation from cells that express different Rad52-FLAG mutants. The anti-Dps1 antibody was used as loading control. The signals corresponding to immunoprecipitated Rad52 mutants (Blot: αFLAG) were quantified relatively to the signal for WT Rad52, corrected to the quantity of Rad52 in each extracts (Extracts: αFLAG) and to the Dps1 signal (αDps1). Data are presented as the mean ± SEM (n = 3). Fisher’s exact test, n.s. p>0.05, *p<0.05, **p<0.01, ***p<0.001. (B) Purification of recombinant Rad52-FLAG, Rad52-P381S-FLAG and Rad52-Y376A-FLAG. (C) Pull-down experiments using Rad52-FLAG WT or Y376A fusion protein in the presence of different salt concentrations.

Figure 2—source data 1. Relative IP (%) and Two-tailed p values, unpaired T test for Figure 2A.
elife-32744-fig2-data1.xlsx (42.2KB, xlsx)
DOI: 10.7554/eLife.32744.007