Figure 3. Effect of the rad52-P381S and rad52-Y376A alleles on HR.

(A) Recombination system allowing the formation of a WT ARG4 allele by gene conversion of the arg4-RV or arg4-Bg heteroalleles in diploid cells. (B, C) Survival curves (black) and heteroallelic HR frequencies (red) for the indicated homozygous diploid cells exposed to γ-rays. Data are presented as the mean ± SEM of at least three independent experiments. (D) HO-induced gene conversion between MAT ectopic copies. Cell viability after DSB formation is shown as the mean ± SEM of at least three independent experiments. Fisher’s exact test, n.s. p>0.05, *p<0.05.