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. 2018 Jun 26;7:e36306. doi: 10.7554/eLife.36306

Figure 5. The motile properties of the fully reconstituted mRNP are similar to dynein-dynactin complexes reconstituted with Drosophila DDBCC1 or mammalian BicD2N.

(A) Kymograph of (left panel) a minimal dynein-dynactin-BicDCC1 (DDBCC1) complex, and (right panel) a complex of dynein, dynactin, full-length Drosophila BicD, Egl and K10 mRNA, visualized with a Qdot on BicD (B) Speed distributions of DDBE + K10 mRNA (red circles) (0.42 ± 0.22 μm/s, n = 505) and DDBCC1 (blue squares) (0.43 ± 0.26 μm/s, n = 516, p=0.25, t-test, mean ± SD). (C) Run length distributions of DDBE + K10 mRNA (red circles) (6.4 ± 0.25 μm, n = 71) and DDBCC1 (blue squares) (5.3 ± 0.17 μm, n = 66, p=0.8, Kolmogorov–Smirnov test, mean ±SE of the fit). (D) Speed distributions of DDBE + K10 mRNA (red circles) (0.45 ± 0.21 µm/s, n = 1126) and DD(BicD2N) (blue squares) (0.35 ± 0.19 µm/s, n = 1147; p<0.05, t-test, from 50 representative run trajectories for each condition, mean ± SD). (E) Run length distributions of DDBE + K10 mRNA (red circles) (7.2 ± 0.5 μm, n = 142) and DD(BicD2N) (blue squares) (5.4 ± 0.7 μm, n = 137, p=0.053, Kolmogorov–Smirnov test, mean ± SE of the fit). See Figure 5—source data 1.

Figure 5—source data 1. Dataset for Figure 5.
elife-36306-fig5-data1.xlsx (11.7KB, xlsx)
DOI: 10.7554/eLife.36306.019

Figure 5.

Figure 5—figure supplement 1. Kymograph of (left panel) a minimal dynein-dynactin-BicD2N complex, visualized with a Qdot bound to BicD2N, and (right panel), a complex of dynein, dynactin, full-length Drosophila BicD, Egl and K10 mRNA labeled with Alexa Fluor 488-UTP.

Figure 5—figure supplement 1.

The straight vertical line at the left of both kymographs shows complex accumulation at the minus-end of the microtubule.