Table 2. Oligos used for dsRNA production.
| Gene | Oligonucleotide 1 (5’→3’) | Oligonucleotide 2 (5→3’) | Template | Concentration (mg/ml) |
|---|---|---|---|---|
| cyk-4 (K08E3.6) | CGTAATACGACTCACTATAGGTGTCA AAGACACTCAGAAAC |
CGTAATACGACTCACTATAGGCCTC TTCGAATTGGCAGCAGC |
N2 cDNA | 2.0 |
| zen-4 (M03D4.1) | AATTAACCCTCACTAAAGGAATTGGT TATGGCTCCGAGA |
TAATACGACTCACTATAGGATTGGA GCTGTTGGATGAGC |
N2 cDNA | 1.3 |
|
ect-2
(T19E10.1) |
TAATACGACTCACTATAGGTCTCCGA TAAATCTGTGGGG |
AATTAACCCTCACTAAAGGCAGCAG TTTGCGAAAATGAA |
N2 genomic DNA | 2.0 |
|
spd-1
(Y34D9A.4) |
TAATACGACTCACTATAGGTCGTTGA CGCGTACTCAACT |
AATTAACCCTCACTAAAGGGAATTC GAAATCCGACTCCA |
N2 cDNA | 1.8 |
|
rga-3/4
(K09H11.3/Y75B7AL.4) |
TAATACGACTCACTATAGGCCTTCCT GAGCACGACTTTC |
AATTAACCCTCACTAAAGGAGCTTT CGCGACCTTAAACA |
N2 genomic DNA | 2.6 |
| rho-1 (Y51H4A.3) | AATTAACCCTCACTAAAGGATCGTC TGCGTCCACTCTCT |
TAATACGACTCACTATAGGCTCGGC TGAAATTTCCAAAA |
N2 genomic DNA | 1.9 |
| ced-10 (C09G12.8) | AATTAACCCTCACTAAAGGATCGCC TCATCGA AAACTTG |
TAATACGACTCACTAT AGGTCAAAT GTGTCGT CGTTGGT |
N2 cDNA | 2.0 |
| cdc-42 (R07G3.1) | AATTAACCCTCACTAAAGGGTTTGG CATTTTTCAGGGAA |
TAATACGACTCACTATAGGACGTGT GCGTGCACATTTAT |
N2 genomic DNA | 2.0 |
| hyls-1 (C05C8.9) | AATTAACCCTCACTAAAGGTGGCA AATTTTACCACTGAAA |
TAATACGACTCACTATAGGTGATATC TTGTGACCGGATCA |
N2 cDNA | 2.0 |
| gfp | AATTAACCCTCACTAAAGGCCAA CACTTGTCACTACTTTCTGTTATGG |
TAATACGACTCACTATAGGGTATAGT TCATCCATGCCATGTGTAATCCC |
Plasmid | 2.0 |