Table 1. Imaging and Screening Statistics.

Exemplar resolution and imaging times for given pixel binning levels, using a 10 × 0.5 NA objective and imaging our Tg(mbp:EGFP) reporter line. Fish loading times by LP Sampler to VAST BioImager based on three fish per well (experiment averages 52 s for first fish and 17 s for subsequent two per well). Combined loading and imaging rates per fish for different imaging parameters.

Pixel binning	1 × 1	2 × 2	3 × 3
Pixel Size (nm)	283	566	849
Exposure (ms)	180	45	20
Approximate Imaging Time (s/fish)	260	80	60
Fish loading time (s/fish)	30	30	30
Load and Image Rate (fish/hour) 5 tiles	12	33	40
Load and Image Rate (fish/hour) 1 tiles	43	78	85