Table 2. Salt bridges between formin FH2 domains and actin subunits.
Percentage of the time that salt bridges formed between the lasso and linker regions of Cdc12, Bni1 and mDia1 FH2 domains and actin subunits during the last 20 ns of AA simulations spanning 200 ns of seven-mer filaments.
| FH2 residue | FH2 domain | Actin residue | Actin subunit | % Formed |
|---|---|---|---|---|
| Cdc12/actin | ||||
| R990 | FHL-lasso | D363 | A2 | 100. |
| K992 | FHT-lasso | E125 | A1 | 99.6 |
| K1038 | FHL-linker | E99 | A2 | 71.5 |
| K1041 | FHL-linker | E100 | A2 | 52.5 |
| K1045 | FHL-linker | E2 | A2 | 72.3 |
| K1045 | FHL-linker | D3 | A2 | 93.2 |
| K1046 | FHL-linker | E4 | A2 | 50.3 |
| K1038 | FHT-linker | D363 | A1 | 20.6 |
| Bni1/actin | ||||
| K1357 | FHL-lasso | D363 | A2 | 85.2 |
| K1357 | FHT-lasso | D363 | A1 | 23.0 |
| K1359 | FHT-lasso | E125 | A1 | 99.2 |
| R1402 | FHL-linker | E99 | A2 | 99.4 |
| E1403 | FHL-linker | K359 | A2 | 95.0 |
| K1410 | FHL-linker | E4 | A2 | 24.0 |
| K1410 | FHL-linker | E100 | A2 | 81.8 |
| K1412 | FHL-linker | D3 | A2 | 57.5 |
| R1402 | FHT-linker | D363 | A1 | 98.4 |
| mDia1/actin | ||||
| R764 | FHL-lasso | D363 | A2 | 23.4 |
| K807 | FHL-linker | E125 | A2 | 49.9 |
| K813 | FHL-linker | E4 | A2 | 37.9 |
| K813 | FHL-linker | E99 | A2 | 20.4 |
| K826 | FHL-linker | D3 | A2 | 70.9 |
| K826 | FHL-linker | E2 | A2 | 43.3 |
| K827 | FHL-linker | E2 | A2 | 13.2 |
| K828 | FHL-linker | E2 | A2 | 11.4 |
| K807 | FHT-linker | E125 | A1 | 23.0 |
| K810 | FHT-linker | E83 | A1 | 43.7 |
| K810 | FHT-linker | E125 | A1 | 46.7 |
| K813 | FHT-linker | D51 | A1 | 99.0 |
| E816 | FHT-linker | R37 | A1 | 96.8 |
| K828 | FHT-linker | E2 | A3 | 25.1 |