Table 2.
H2O2-Responsive Probes
| Name | Species tested for cross-reactivity | Reported staining concentration for cells | Time needed before optimal signal available | λex/λem (nm) | References |
|---|---|---|---|---|---|
| Boronate-based probes | |||||
 | |||||
| PAM-BN-PB | CH3COOH, TBHP, ClO−, O2·−, ·OH, −OtBu, GSH, Vc, ONOO−, H2O2 | 10 μM | 30–60 min | 410/480 | (25) |
| HP-L1 | K+, Na+, Ca2+, Mg2+, Zn+, Cu2+, Fe2+, GSH, Cys, HClO, H2O2 | 5 μM | 30 min | 520/584 | (100) |
| Mito-H2O2 | HClO, O2·−, −OtBu, ·OH, TBHP, NO, ONOO−, GSH, Vc, Glu, HSO3−, H2O2 | 10 μM | 30–90 min | 490/527 | (171) |
| QCy-BA | OCl−, O2·−, TBHP, ·OH, −OtBu, NO, ONOO−, H2O2 | 5 μM | 30 min | 400/565–680 | (116) |
| PF1 | OCl−, O2·−, TBHP, ·OH, −OtBu, NO, H2O2 | 5 μM | 5 min | 450/460–700 | (15) |
| TPE-DABA (AIE) | OCl−, O2·−, TBHP, ·OH, −OtBu, NO, NO3−, ONOO−, H2O2 | 50 μM | N/A | 430/576 | (98) |
| TPE-BO (AIE) | 1O2, NO, ClO−, TBHP, ·OH, O2·−, GSH, H2O2 | 5 μM to 1.0 mM | N/A | 400/500 | (189) |
| D-BBO (AIE) | 1O2, NO, ClO−, TBHP, ·OH, O2·−, H2O2 | N/A | N/A | 405/510 | (86) |
| Benzil-based probes | |||||
 | |||||
| NBzF-BG | N/A | 2 μM | 10 min | 505/525 | (1) |
| NBzF | 1O2, ONOO−, NO, ClO−, TBHP, ·OH, O2·−, H2O2 | 5 μM | 10 min | 495/519 | (2) |
| Chalcogenides-based probes | |||||
 | |||||
| 2-Me TeR | ONOO−, NO, ClO−, ·OH, O2·−, H2O2 | 5 μM | N/A (short lived) | 660/690 | (78) |
| Compound 3 | N/A | N/A | N/A | 500/565 | (74) |
| Metal-mediated redox probes | |||||
 | |||||
| ZP1Fe2 | CAN, DDQ, ClO−, O2·−, −OtBu, ·OH, TBHP, NO, H2O2 | 10 μM | 30 min | 507/533 | (139) |
| MBFh1 | N/A | 5 μM | 1 min | 570/590 | (62) |
| Other probes | |||||
| 1a | ONOO−, NO, TBHP, ClO−, ·OH, O2·−, O2·−+catayse, O2·−+SOD, H2O2 | 25 μM | 60 min | 485/530 | (106) |
 | |||||
| DA-Cy | ·OH, MeLOOH, CuOOH, tBuOOH, O2−, 1O2, ClO, H2O2 | 5 μM | 3 min | 630/755 | (180) |
AIE, aggregation induced emission; N/A, not available; NO, nitric oxide; O2·−, superoxide; OCl−, hypochlorite; OH·, hydroxyl radical; ONOO−, peroxynitrite.