Table 3.

Peroxynitrite-Responsive Probes

Name	Species tested for cross-reactivity	Reported staining concentration for cells	Time needed before optimal signal available	λex/λem (nm)	References
Addition-based probes
A2	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, NO2−, 1O2	2 μM	20 min	485/517	(109)
Ds-DAB	OCl−, O2·−, ·OH, NO, NO3−, ONOO−, H2O2, NO2−, HNO	10 μM	30 min	350/505	(91)
MITO-CC	OCl−, O2·−, ·OH, NO, NO3−, ONOO−, H2O2, NO2−, TBHP, BuO·, H2S, H2S2, SO32−, HNO	5 μM	20 min	420/651	(27)
CHCN	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, Cys, Hcy, GSH, TBHP	5 μM	30 min	515/635	(192)
HKGreen-1	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, 1O2	20 μM	15 min	490/521	(173)
HKGreen-2	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, 1O2, NO, NO3−	20 μM	60 min	520/539	(142)
Oxidation-based probes
HKGreen-4	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, 1O2	10 μM	60 min	517/535	(122)
NP3 (ESIPT)	OCl−, O2·−, ·OH, ROO−, NO, ONOO−, H2O2, 1O2, Ala, Gly, Cys, GSH, Cu2+, Fe2+, Fe3+, Zn2+	5 μM	30 min	405/420–480	(88)
1-D-fructose	OCl−, O2·−, ·OH, ROO−, NO, NO3−, ONOO−, H2O2, NO2−,	5 μM	30 min	410/525	(141)
PyBor	OCl−, O2·−, ·OH, NO, ONOO−, H2O2, TBHP, CuOOH, BrO	10 μM	30 min	347/410	(182)
Fl-B	N/A	50 μM	30 min	492/515	(128)
Cy-NTe	OCl−, O2·−, ·OH, NO, ONOO−, H2O2, TBHP, CuOOH, MeLOOH	1 μM	5 min	793/820	(181)
Other probes
5-Fluoroisatin	Detected by 19F NMR				(13)
PN600	Three-channel fluorescent probe				(188)

Cys, cysteine; ESIPT, excited state intramolecular proton transfer; ¹O₂, singlet oxygen.