Table 5.

Thiol-Responsive Probes

Name	Species tested for cross-reactivity	Reported staining concentration for cells	Time needed before optimal signal available	λex/λem (nm)	References
Reversible reaction-based probes
RealThiol	GSH, Gly, Lysate, Cys, H2S, Lipoic acid, KSCN, Na2S2O3, NO, H2O2, HOCl, O2−, NaNO2, Protein thiols	0.1–1 μM	1–5 min	405/487	(66)
				488/565
TQ-Green	GSH, Cys, BSA, Lysate	1 μM	15–30 min	405/488	(67)
				488/565
QG-1	H2O2, GSH	10 μM	1 min	405/488	(101)
				488/560
QG3.0	H2O2, GSH	1 μM	1–5 min	558/593	(151)
				609/632
Irreversible reaction-based probes
Probe 1	Glu, Ala, Arg, Val, Ser, Lys, Leu, Adenine, Guanine, Cytosine, Thymine, Glucose, Ca2+, Mg2+, K+, Na+, Zn2+, Fe3+, NADH, H2O2	N/A	N/A	444/496	(93)
1	Ca2+, Mg2+, K+, Na+, Zn2+, Fe3+, Cu2+, Hg2+, GSH	1 μM	5 min	400/420–600	(175)
Probe	DTT, 3′-thio dT, Glu, Ala, Arg, Lys, ME, Vc, H2O2	25 μM	15 min	490/522	(135)
Aldehyde 1	Glu, Ala, Arg, Ser, Lys, Leu, Pro, His, Gln, GSH, Cys	N/A	N/A	315/360	(143)