Table 1. Murine-specific primers.
Murine-specific primers designed using MacVector for real time PCR.
| Molecule (gene) | Mouse accession # | Sequence 5′ to 3′ | Annealing temp (°C) | Product size (bp) |
|---|---|---|---|---|
| Extracellular matrix proteins | ||||
| Collagen I (Col1a1) | X06753 | F TCT CCA CTC TTC TAG TTC CT R TTG GGT CAT TTC CAC ATG C |
55 | 269 |
| Versican (Vcan) | XM994074 | F ATG ATG GGG AAG GAA GGG GTT C R AGC CAG CCG TAA TCG CAT TG |
57 | 236 |
| Biglycan (Bgn) | L20276 | F ACT TCT GTC CTA TGG GCT TCG G R GCT TCT TCA TCT GGC TAT GTT CCT C |
57 | 218 |
| Lumican (Lum) | NM008524 | F TAC AAC AAC CTG ACC GAG TCC G R CGA GAC AGC ATC CTC TTT GAG C |
55 | 159 |
| Decorin (Dcn) | NM007833 | F CAA CAA CAA ACT CCT CAG GGT GC R TTG CCG TAA AGA CTC ACA GCC G |
57 | 165 |
| Elastin and associated proteins | ||||
| Perlecan (Hspg2) | NM008305 | F TCT GTC TGC CTG GCT TCT CT R CGA ATT CAA TTG TCT CGG GT |
56 | 204 |
| LTBP-1 (Ltbp1) | NM019919 | F GGG AGC ATC TGA GTG AGG AG R TCA CAG GGA TAT TGC ACA GC |
56 | 165 |
| LTBP-2 (Ltbp2) | NM013589 | F CAC CCA GAC CAG CCT TCC CA R AGT CCT TGC AGA GGC CCA GG |
57 | 126 |
| Fibrillin-1 (Fbn1) | NM007993 | F ATC CGC TGT ATG AAT GGG GG R CTG GCA CAT CTG GTT GCT TAC C |
58 | 228 |
| Elastin (Eln) | NM007925 | F AGC CAA ATA TGG TGC TGC TG R GGG TCC CCA GAA GAT CAC TT |
58 | 246 |
| MMPs/TIMPs | ||||
| MMP-2 (Mmp2) | NM008610 | F ATT TGG CGG ACA GTG ACA CCA C R ATC TAC TTG CTG GAC ATC AGG GGG |
59 | 231 |
| MMP-3 (Mmp3) | NM010809 | F GCT GAG GAC TTT CCA GGT GTT G R GGT CAC TTT TTT GGC ATT TGG GTC |
53 | 120 |
| MMP-13 (Mmp13) | NM008607 | F GAT GAC CTG TCT GAG GAA G R ATC AGA CCA GAC CTT GAA G |
55 | 357 |
| ADAMTS-4 (Adamts4) | NM172845 | F TAA CTT GAA TGG GCA GGG GGG TTC R AAT GGC TTG AGT CAG GAC CGA AGG |
60 | 245 |
| TIMP-1 (Timp1) | BC051260 | F ATC TCT GGC ATC TGG CAT CCT C R GGT GGT CTC GTT GAT TTC TGG G |
56 | 154 |
| TIMP-3 (Timp3) | NM011595 | F ATT ACC GCT ACC ACC TGG GTT G R TCT GGG AAG AGT TAG TGT CTG GGA C |
58 | 198 |
| GAPDH (Gapdh) | BC083149 | F TGC GAC TTC AAC AGC AAC TC R CCT GCT CAG TGT CCT TGC TG |
55 | 200 |