Fig. 5:

Effect of bortezomib-loaded anti-CD38 chitosan NPs on proliferation, cell cycle, and apoptosis. The effect of vehicle (control), BTZ as a free drug (5 nM), empty chitosan NPs, non-targeted chitosan NPs loaded with BTZ equivalent amounts to 5 nM, and anti-CD38 chitosan NPs loaded with BTZ equivalent amounts to 5 nM for 48 h on: A) proliferation of MM cells and PB MNCs analyzed by MTT, *p<0.05; B) % Sub-G1 population of H929 cells measured by Propidium Iodide stained of DNA, *p<0.05; C) Apoptosis of MM cells by FITC-Annexin-V and Propidium Iodide, *p<0.05. D) Survival–associated molecules (pERK and pAKT) after 6h of treatment, cell cycle–associated molecule (pRB), as well as, apoptotic–associated molecules (cleaved PARP and cleaved caspase 3) after for 12h of treatment, in H929 cells were measured by immunoblotting.