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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Surgery. 2018 May 3;164(2):262–273. doi: 10.1016/j.surg.2018.03.008

Figure 7. GDF11 induces activation and proliferation of renal fibroblasts.

Figure 7

(A) MTT dye reduction assay of NRK49f murine renal fibroblasts after 5 d of culture with the indicated dose of recombinant GDF11 (white) or TGF-β (grey). (B) Western blot analysis of αSMA and collagen I (Col I) in extracts from NRK49f cells starved for 48 h before treatment with recombinant TGF-β or GDF11 for 18 h. GAPDH is a loading control. (C) Immunohistochemistry for αSMA in NRK49f cells grown in chamber slides and treated for 48 h with carrier containing no growth factor or with 50 ng/mL GDF11. **P < 0.01, ***P < 0.001.