. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: Surgery. 2018 May 3;164(2):262–273. doi: 10.1016/j.surg.2018.03.008

Table 2.

Primers for qRT-PCR analysis

18S rRNA	Forward	5’- CATGTGGTGTTGAGGAAAGCAG-3’
18S rRNA	Reverse	5’- TCTTGTACTGTCGTGGGTTCTG-3’
Acta2 (αSMA)	Forward	5’- ACTGGGACGACATGGAAAAG-3’
Acta2 (αSMA)	Reverse	5’- CATCTCCAGAGTCCAGCACA-3’
Bmp1	Forward	5’- CAGGCAACCGCATGTTCTT-3’
Bmp1	Reverse	5’- GTGGGATGCCTGGAAACCT-3’
Bmp7	Forward	5’- TGTGGCAGAAAACAGCAGCA-3’
Bmp7	Reverse	5’- TCAGGTGCAATGATCCAGTCC-3’
Coll o	Forward	5’-AAAGGCTGGAGAGCGA-3’
Coll o	Reverse	5’- AGCAGGACCTGGGGGA-3’
Col3a1	Forward	5’- TGGTCCTCAGGGTGTAAAGG-3’
Col3a1	Reverse	5’- GTCCAGCATCACCTTTTGGT-3’
Col4a3	Forward	5’- CCCAGCCAGTCCATTTATAGAAT-3’
Col4a3	Reverse	5’- CAGCGAAGCCAGCCAGAA-3’
Cadh1 (E-cadherin)	Forward	5’- AAGAAGACCAGGACTTTGATTTGAGCCAGCTGC-3’
Cadh1 (E-cadherin)	Reverse	5’- TTCAGAACCACTGCCCTCGTAATCGAACACCAACAG-3’
Gapdh	Forward	5’-TGCACCACCAACTGCTTAG-3’
Gapdh	Reverse	5’-GGATGCAGGGATGATGTTC-3’
Tgfb1	Forward	5’- GCAACATGTGGAACTCTACCAGA-3’
Tgfb1	Reverse	5’- GACGTCAAAAGACAGCCACTCA-3’
Vim (Vimentin)	Forward	5’- ATGCGTGAGATGGAAGAGAATTTTGC-3’
Vim (Vimentin)	Reverse	5’- TTATTCAAGGTCATCGTGATGCTGAGA-3’