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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Bone. 2018 Jun 5;114:22–31. doi: 10.1016/j.bone.2018.06.002

Fig. 5. Increased serum bone resorption markers in young and old mice, and osteoclast differentiation in vitro induced by mefloquine.

Fig. 5

(A) Osteoclast number and (B) genes associated with osteoclast differentiation in cultures of non-adherent bone marrow cells treated with M-CSF and sRANKL, measured by qPCR and corrected by GAPDH (n=4). Representative images of in vitro generated osteoclasts are shown. Scale bars indicate 10μm. (C) ATP levels were measured in osteoclast lysates (intracellular ATP, n=3) after 7 days of differentiation using a luciferin-luciferase kit. Mature osteoclasts treated with vehicle or 1mM mefloquine for 24h were stained using 100μM quinacrine and visualized under fluorescence microscope (n=3). Scale bars indicate 100μm. Bars represent mean ± s.d. *p<0.05 versus vehicle treated group, by t-test.