Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Bone. 2018 Jun 5;114:22–31. doi: 10.1016/j.bone.2018.06.002

Fig. 6. Mefloquine administration does not affect sclerostin levels or osteocyte apoptosis in young or old mice.

Fig. 6

(A) Sost mRNA was measured in mefloquine-treated young and old mice in tibia (n=8–10). (B) sclerostin protein levels were measured by ELISA in L5 vertebra lysates (n=8–10). (C) Osteocyte numbers were measured in cortical bone of the femur and cancellous bone of the L3 vertebra (n=8–10). (D) Apoptotic osteocytes (active caspase-3-positive) and empty lacunae were enumerated in the femoral mid-diaphysis of young and old mice (n=5–9). Representative immunostaining image with a typical active caspase-3-positive (black arrow, brown) and viable osteocyte (white arrow, blue-green) is shown. (E) Genes associated with apoptosis were measured in tibiae without bone marrow cells at 3.5 and 21 months of age by qPCR and corrected by GAPDH (n=6–10).