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. Author manuscript; available in PMC: 2019 Jul 23.
Published in final edited form as: Cancer Immunol Res. 2018 Jan 23;6(3):305–319. doi: 10.1158/2326-6066.CIR-16-0358

Figure 2. Generation of spontaneous HPV buccal tumors in immunocompetent mice.

Figure 2

(A) C57BL/6NCr, Balb/c, or FVB/NCr mice (n=5/group) received submucosal plasmid injections, followed by electroporation. The plasmids contained HPV16-E6/E7, luciferase, NRasG12V, and SB100 transposase. Bioluminescence intensity was recorded by an IVIS Spectrum. (B) Schematic diagram of treatment protocol. Control C57BL/6NCr mice (n=5): plasmid transfection only; CD3-depleted C57BL/6NCr mice (n=5): 100 μg anti-CD3 IP for a total of three doses at one-day intervals before plasmid transfection; CD4-depleted C57BL/6NCr mice (n=5): began with 100 μg anti-CD4 IP for a total of three doses at one-day intervals before receiving one dose weekly after plasmid transfection; CD8-depleted C57BL/6NCr mice (n=5): began with 200 μg anti-CD8 IP for a total of three doses at one-day intervals before receiving two doses weekly after plasmid transfection. (C) Bioluminescence kinetics of buccal tumors in different groups. Three independent experiments were performed. Data are presented as mean ± SD.