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. 2018 Jul 23;8:11097. doi: 10.1038/s41598-018-29378-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methylation of the 5′-associated region of VWA2 in CRC cell lines. On top, a scheme of the region. Symbols and tracks identical to those in Fig. 1. At the bottom, bisulfite sequencing results. Analyses were performed in 4 separate PCRs. Every line represents and individually sequenced clone. Dots represent unmethylated (blue) or methylated (black) CpG sites. Primary cancer-derived cell lines are organized from higher (HT29) to lower (SW480) methylation levels. The lymph node-derived cell line SW620 is shown at the bottom.