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. 2018 Jul 23;8:11056. doi: 10.1038/s41598-018-29212-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological examination of tumors from hiPSCs and EB cells. Representative microscopic images of Haematoxylin & Eosin-stained sections of (A) hiPSCs derived-teratoma showing the differentiation into all three germ layers; (B,C) mature and immature cartilage (mesoderm); (D,E) primitive neuroectodermal tissue (endoderm) in the form of rosettes with high mitotic activity (arrowheads); (F) cellular primitive stromal tissue; (G) hemorrhage and necrosis (asterisk); (H,I) glandular and columnar epithelial tissue (endoderm); (J,L) a small lymph node associated with fibrosis and edema was found in the site of inoculation of differentiated EBs (20 days).