Table 1.
Experimental pipeline.
| Cells line | Method of generation | Method of cell detachment and dissociation | Number of mice | Site and number of cells per injection | Teratoma | Time of teratoma formation | Method of analysis |
|---|---|---|---|---|---|---|---|
| hiPSCs-P1 hiPSCs-V2 |
hSTEMCCA lentiviral vector |
Collagenase IV/accutase solution | 5 CD1 Nude | subcutaneous 10 × 106 |
Immature teratoma Grade 3 |
4–10 weeks | GC/MS gas sensors H&E |
| EBs at day 5 | floating | Trypsin-like enzyme (TrypLE) | 5 CD1 Nude | subcutaneous 5 × 106 |
lymph nodes, fibrosis and edema in the site of inoculation | GC/MS gas sensors H&E |
|
| differentiated cells | spontaneous | Trypsin | 5 CD1 Nude | subcutaneous 5 × 106 |
Lymph nodes, fibrosis and edema in the site of inoculation | GC/MS gas sensors H&E |
|
| Placebo | Matrigel | 5 CD1 Nude | subcutaneous 100 µl |
GC/MS gas sensors |