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. 2018 Jul 23;8:11025. doi: 10.1038/s41598-018-29068-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Variation in transverse grain measurements observed across 33 barley genotypes. (A) Box plot of normalised data showing the variation in different grain measurements. (B) Frequency distribution plots of the four aleurone measurements. (C) Principal Component Analysis separates the genotypes based on the seven transverse measurements (variables). EA, endosperm area; AA, aleurone area; AP, aleurone proportion; DVW, grain dorsal-ventral distance; LRW, transverse grain left-right width; ALN, aleurone layer number; AW, aleurone width.