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. 2018 Jul 23;8:11025. doi: 10.1038/s41598-018-29068-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transverse and wholegrain trait correlations across different barley genotypes. (A–C) Phenotypic measurements from all grain samples for 33 genotypes obtained using transverse grain sections. (A) Aleurone area vs endosperm area. (B) Aleurone proportion vs endosperm area. (C) Aleurone width vs aleurone area. (D–F) Correlations between grain measurements (averages) for all 2-row genotypes (n = 30) using the SeedCount, Single Kernel Characterisation System and/or transverse grain sections. (D) Grain width vs endosperm area. (E) Grain weight vs endosperm area. (F) Grain hardness index vs aleurone proportion. EA, Endosperm area; AA, aleurone area; AP, aleurone proportion; AW, aleurone width; GWi, grain width; GWt, grain weight. Significance indicators: ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05.