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. 2018 Jul 23;8:11025. doi: 10.1038/s41598-018-29068-4

Figure 5.

Figure 5

Accumulation of transcript in different barley grain tissues. (A) RNAseq analysis of transcripts from Sloop wholegrain samples, minus embryo, at 7, 9, 11, 13, 15, 20 days post anthesis (DPA). Accumulation patterns for the HvBMY1, HvBMY2 and HvLTP2 transcripts are shown, normalised to the maximum expression value for each gene. (B) Thin butyl-methyl methacrylate sections of Sloop barley grain at 11 DPA showing the regions collected by laser microdissection. PE, pericarp; AL, aleurone; OSE, outer starchy endosperm including sub-aleurone; ISE, inner starchy endosperm. The red dashed box shows a magnified view of the outer grain layers. (CE) Quantitative PCR analysis of transcript abundance in RNA collected from laser micro dissected material. (C) lipid transfer protein 2 (HvLTP2). (D) hordoinoline a (HvHINa) (E) β-amylase 1 (HvBMY1) and β-amylase 2 (HvBMY2).