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. 2018 Jul 23;8:11038. doi: 10.1038/s41598-018-29350-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TIDE analysis of Cas9/sgRNA-stimulated EML4-ALK junctions. (A) Representative primary sequence data. Top panel, amplification of cloned DNA representing exact joining of Cas9/sgRNA-generated blunt ends. Bottom panel, amplification of pooled genomic DNA from a flask of HBEC-3KT F25F cells irradiated with 1.0 Gy of 108 keV/μm ⁴⁸Ti ions and subjected to Cas9/sgRNA challenge. (B) TIDE analyses of DNA from multiple independent cell populations. Samples are from the same experiment as in Fig. 2A. Red bars denote recurrent deletions; grey bars denote exact joining. Only peaks scored as significant are shown. Contribution of each species to the total is indicated (%). Percentages sum to less than 100 because non-significant peaks are not scored or shown.