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. 2018 Jul 23;4:81. doi: 10.1038/s41420-018-0083-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–c DHE staining of freshly dissected hindgut. a′–c′ are DHE channel. Asterisk denotes potentially non-apoptotic cell death in opa1RNAi hindguts. d–f Anti-beta Gal staining against Puc-LacZ in different genotypes. g–i Differentiation index, such as Stat::GFP and gut length, was compared in different conditions. Gut length was delineated out by white lines. Scale bar is 20 µm