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. 2018 Jul 23;8:11089. doi: 10.1038/s41598-018-29432-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetic analysis of ALP activity in plasma samples before and after dialysis. (A) Representative Michaelis-Menten saturation curves to determine the K_m and V_max of pNPP hydrolysis in pre- and post-dialysis plasma samples under physiological conditions (top). Representative Lineweaver-Burk plot (bottom). The curves for each patient were used to determine V_max (B) and K_m (C) using nonlinear regression, as described in the Methods section. Results are presented as mean ± SEM (n = 40), and were compared by the Wilcoxon matched pairs test. *P < 0.05; ***P < 0.001.