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. 2018 Jul 23;8:11089. doi: 10.1038/s41598-018-29432-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pyrophosphate hydrolysis in plasma is higher in patients after than before dialysis. Plasma samples obtained before and after dialysis were incubated for for the indicated time with 5 μmol/L pyrophospahte and 32-pyrophosphate (³²PPi) as a radiotracer. A 5 μL aliquot of each sample was separated by thin layer chromatography. After radiography, the spots were excised and added to liquid scintillation fluid. Pyrophosphate hydrolysis was quantified as percent 32-phosphate (³²Pi) produced relative to total CPM (³²Pi + ³²PPi) and represented as fmol pyrophosphate hydrolyzed per hour and per μL of plasma sample (fmol*hour⁻¹*μL⁻¹). (A) Pyrophosphate hydrolysis in post-hemodialysis plasma for the indicated time. Results are presented as mean ± SEM (n = 9). (B) Representative radiography of a thin layer chromatograph showing ³²Pi and ³²PPi after incubation for 4 hours. (C) Pyrophosphate hydrolysis in plasma samples before (PreHD) and after (PostHD) dialysis after incubation for 4 hours. Results are presented as mean ± SEM (n = 40), and were compared by the Wilcoxon matched pairs test. ***P < 0.001.